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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which ends up in chromosome instability, and thus may lead to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant role in advertising cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two extra splice variants have been claimed but in-depth nucleotide sequence details will not be yet out there.
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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually has the epitope regarded via the antibody.
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This manual outlines further more probable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to make certain the very best final results are...
Antibodies certain to the blocking peptide not bind read more into the epitope about the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, you can see which staining is unique.
Focus on Info Cyclin E belongs towards the remarkably conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which contribute to your temporal coordination of each mitotic party. Cyclin E kinds a posh with and features as a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary which is degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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